Autoimmune thyroid gland disorders are characterized by detection of anti-thyroid
antibodies, primarily against thyroglobulin and/or microsomal thyroid antigens.
Recently it has been shown that thyroid peroxidase (TPO) is the protein responsible
for microsomal antigenicity. In addition to chronic thyroiditis, thyroid
autoantibodies may be found in other thyroid disorders. These autoantibodies may
also occur in apparently normal subjects.
Thyroid microsomal (TPO) autoantibodies occur in sera of most autoimmune thyroid
disease patients and predict raised serum TSH levels in random populations.
The presence of autoantibody does not imply active tissue destruction. Microsomal (TPO)
antibody level correlates with the degree of lymphoid infiltration of the thyroid gland.
Thyroid autoantibody level correlates with the degree of lymphoid infiltration of
the thyroid gland. It is also reported that low levels of autoimmune antibodies predict
at-risk pregnancy. Furthermore, an EIA test for anti-thyroglobulin and anti-recombinant
TPO demonstrated an increase in the rate of spontaneous miscarriage in women who had
detectable serum thyroid autoantibodies in their first trimester of pregnancy.
Thyroid autoantibodies are detected by a variety of immunoassays. Common methods are
enzyme immunoassay (EIA), indirect hemagglutination (IHA) and indirect fluorescent
antibody (IFA) techniques.
GenBio offers thyroid autoimmunity screening test in both the ImmunoDOT and the
ImmunoWELL test formats.
ImmunoDOT Thyroid Autoimmunity Test simultaneously detects both thyroid microsomal
(thyroid peroxidase) autoantibodies and thyroglobulin autoantibodies using highly purified
human thyroglobulin devoid of microsomal antigen and recombinant human thyroid peroxidase
(microsomal antigen) which does not contain contaminating thyroglobulin and/or mitochondria
found in other microsome antigen preparations.
The ImmunoWELL Microsome (TPO recombinant) Antibody Test and the ImmunoWELL Thyroglobulin
Antibody Test are enzyme immunoassays (EIA) for screening and detection of autoantibodies
against human thyroid peroxidase (microsome) and human thyroglobulin in serum as an aid in
the diagnosis of thyroid disorders.